Translational_Unit

Part:BBa_K2090001

Designed by: TP_CC-SanDiego 2016   Group: iGEM16_TP_CC_SanDiego   (2016-10-14)


phoA->LbCHI32->Flag tag

This linked protein encodes a Limonium bicolor-derived LbCHI32 chitinase enzyme with the signal sequence of phoA (alkaline phosphatase) linked upstream of the chitinase gene by a glycine-serine linker. Downstream from the LbCHI32 gene is a FLAG tag used for protein expression and production analysis via immunoprecipitation.

The translated protein encodes a chitinolytic enzyme that can be secreted extracellularly by Escherichia coli; the presence of this protein can be identified using anti-FLAG antibodies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 613


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Categories
Parameters
None